Designed for the measurement of human IgA in serum, plasma, cell culture supernatants, or other biological fluids Easy-to-use enzyme-linked immunosorbent assay (ELISA) Fast results—takes less than two hours to perform Store all kit reagents between 2 and 8°C The IMMUNO-TEK Human IgA ELISA Kit is a rapid, easy to use enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human IgA in serum, plasma, cell culture supernatants or other biological fluids. The assay contains ready-to-use reagents and takes less than two hours to perform. Microwells coated with polyclonal antibodies to human IgA form the capture phase of the assay. Captured human IgA then reacts with detector antibody which is a polyclonal anti-human IgA conjugated with horseradish peroxidase. Enzyme activity in the wells is then quantified using tetramethyl benzidine as a substrate.