KE00326 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ICOSLG ELISA kit is to be used to detect and quantify protein levels of endogenous human ICOSLG. The assay recognizes human ICOSLG. An antibody specific for human ICOSLG has been pre-coated onto the microwells. The human ICOSLG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ICOSLG is added to detect the captured human ICOSLG protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.