KE00343 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HMGB1 ELISA kit is to be used to detect and quantify protein levels of endogenous human HMGB1. The assay recognizes human HMGB1. An antibody specific for human HMGB1 has been pre-coated onto the microwells. The human HMGB1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HMGB1 is added to detect the captured human HMGB1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.