KE00176 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The gp130 ELISA kit is to be used to detect and quantify protein levels of endogenous gp130. The assay recognizes human gp130. An antibody specific for gp130 has been pre-coated onto the microwells. The gp130 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human gp130 is added to detect the captured human gp130 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.