KE00200 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GNLY/Granulysin ELISA kit is to be used to detect and quantify protein levels of endogenous GNLY/Granulysin. The assay recognizes human GNLY/Granulysin. An antibody specific for GNLY/Granulysin has been pre-coated onto the microwells. The GNLY/Granulysin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GNLY/Granulysin is added to detect the captured GNLY/Granulysin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.