KE00097 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Fetuin A ELISA kit is to be used to detect and quantify protein levels of endogenous Fetuin A. The assay recognizes human Fetuin A. An antibody specific for Fetuin A has been pre-coated onto the microwells. The Fetuin A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Fetuin A is added to detect the captured Fetuin A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.