KE00034 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The F12 ELISA kit is to be used to detect and quantify protein levels of endogenous F12. The assay recognizes human F12. An antibody specific for F12 has been pre-coated onto the microwells. The F12 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for F12 is added to detect the captured F12 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.