KE00344 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Cyclin D1 ELISA kit is to be used to detect and quantify protein levels of endogenous human Cyclin D1. The assay recognizes human Cyclin D1. An antibody specific for human Cyclin D1 has been pre-coated onto the microwells. The human Cyclin D1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Cyclin D1 is added to detect the captured human Cyclin D1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.