KE00271 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CXCL5 ELISA kit is to be used to detect and quantify protein levels of endogenous human CXCL5. The assay recognizes human CXCL5. An antibody specific for human CXCL5 has been pre-coated onto the microwells. The human CXCL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CXCL5 is added to detect the captured human CXCL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.