KE00312 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Clusterin ELISA kit is to be used to detect and quantify protein levels of endogenous human Clusterin. The assay recognizes human Clusterin. An antibody specific for human Clusterin has been pre-coated onto the microwells. The human Clusterin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human Clusterin is added to detect the captured human Clusterin protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.