KE00306 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CEACAM8/CD66b ELISA kit is to be used to detect and quantify protein levels of endogenous human CEACAM8/CD66b. The assay recognizes human CEACAM8/CD66b. An antibody specific for human CEACAM8/CD66b has been pre-coated onto the microwells. The human CEACAM8/CD66b protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CEACAM8/CD66b is added to detect the captured human CEACAM8/CD66b protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.