KE00281 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CEACAM5 ELISA kit is to be used to detect and quantify protein levels of endogenous CEACAM5. The assay recognizes human CEACAM5. An antibody specific for CEACAM5 has been pre-coated onto the microwells. The CEACAM5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for CEACAM5 is added to detect the captured CEACAM5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.