KE00251 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD40 ligand/CD154 ELISA kit is to be used to detect and quantify protein levels of endogenous CD40 ligand/CD154.The assay recognizes human CD40 ligand/CD154 . An antibody specific for CD40 ligand/CD154 has been pre-coated onto the microwells. The CD40 ligand/CD154 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CD40 ligand/CD154 is added to detect the captured human CD40 ligand/CD154 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.