KE00342 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The humanCD40 ELISA kit is to be used to detect and quantify protein levels of endogenous humanCD40. The assay recognizes humanCD40. An antibody specific for humanCD40 has been pre-coated onto the microwells. The humanCD40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for humanCD40 is added to detect the captured humanCD40 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.