KE00190 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD163 ELISA kit is to be used to detect and quantify protein levels of endogenous CD163. The assay recognizes human CD163. An antibody specific for CD163 has been pre-coated onto the microwells. The CD163 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human CD163 is added to detect the captured human CD163 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.