CD16, which is also known as Fc?RIII, is a receptor for the Fc? portion of IgG and is involved in antibody dependent cellular cytotoxicity. 5%-10% human peripheral blood mononuclear cells (PBMC) express CD16. Human CD16 Magnetic Beads Kit is used for isolation or depletion of human CD16 NK cells/monocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD16 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD16+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD16- cells remain in supernatant and can also be used for further applications.