CD14 is a surface antigen expressed on monocyte/macrophage. It cooperates with other proteins to mediate innate immune response to bacterial LPS. 5%-10% human peripheral blood mononuclear cells (PBMC) express CD14. Human CD14 Magnetic Beads Kit is used for isolation or depletion of human CD14 monocytes/macrophages from PBMC, whole blood, or other sample types. CD14 positive cells and negative cells could be separated after short incubation with Human CD14 Magnetic Beads on a magnet. CD14 positive cells remain attached to magnetic beads after separation and can be used for further molecular analysis or in vitro culture, but not suitable for flow cytometry analysis. CD14 negative cells remain in the supernatant and can be used also.