KE00303 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human B2M ELISA kit is to be used to detect and quantify protein levels of endogenous human B2M. The assay recognizes human B2M. An antibody specific for human B2M has been pre-coated onto the microwells. The human B2M protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human B2M is added to detect the captured human B2M protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.