KE00337 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Alpha-1-Antitrypsin/SERPINA1 ELISA kit is to be used to detect and quantify protein levels of endogenous Alpha-1-Antitrypsin/SERPINA1. The assay recognizes human Alpha-1-Antitrypsin/SERPINA1. An antibody specific for Alpha-1-Antitrypsin/SERPINA1 has been pre-coated onto the microwells. The Alpha-1-Antitrypsin/SERPINA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-Alpha-1-Antitrypsin/SERPINA1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.