KE00286 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ADAMTS13 ELISA kit is to be used to detect and quantify protein levels of endogenous human ADAMTS13. The assay recognizes human ADAMTS13. An antibody specific for human ADAMTS13 has been pre-coated onto the microwells. The human ADAMTS13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human ADAMTS13 is added to detect the captured human ADAMTS13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.