KE00130 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The A1BG ELISA kit is to be used to detect and quantify protein levels of endogenous A1BG. The assay recognizes human A1BG. An antibody specific for A1BG has been pre-coated onto the microwells. The A1BG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for A1BG is added to detect the captured A1BG protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.