Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. Intact Genomics Hot Start Taq 2x Master Mix has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with the unique Intact Genomics 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84%
Product Includes:
Hot start Taq 2x master mix
5X Magic Enhancer
• Useful for Routine PCR, Primer Extension, Colony PCR and more;
• Supplied as convenient Master Mix;
• Efficient for amplifying high GC template DNA with Magic Enhancer;
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.