G-biosciences hot start high-fidelity DNA Polymerase is chemically modified that possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This Polymerase has the fidelity, sensitivity and processivity with an error rate ~2.8×102-fold lower than Taq DNA polymerase, and significantly lower than other proofreading DNA polymerases.
Product Includes:
- Hot start high fidelity DNA Polymerase
- 2.5X high fidelity PCR buffer
Applications:
- High fidelity PCR
- Cloning
- Long and high GC content template DNA amplification.
Unit Definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72º C.
Storage Temperature:
- 20ºC