Streamlined host depletion and bacterial DNA isolation from mammalian samples.
- Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA
- Fast: Only 30 minutes of hands-on time for purification of high-quality DNA
- Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile
The HostZERO™ Microbial DNA Kit is designed to overcome the challenge of contaminating host nucleic acids in microbial samples. This kit uses a novel method to reduce the amount of contaminating host DNA by selectively lysing the eukaryotic cells and degrading this DNA prior to total DNA purification. Paired with Zymo Research’s non-biased purification technology, the HostZERO™ Microbial DNA Kit allows for the exclusive capture of DNA from living microbial cells in a biological sample. This new technology is able to reduce the presence of human DNA in a saliva sample from 65% (untreated sample) to less than 1% (treated sample). Concurrently, the depletion process utilizes the ZymoBIOMICS® non-biased DNA isolation technology for accurate data representation. Finally, the HostZERO™ Microbial DNA Kit recovers the highest amount of bacterial DNA as compared to other methods. By removing the presence of host DNA and reducing bias in purification, the HostZERO™ Microbial DNA Kit produces the highest-quality data for microbial samples.