Low to medium throughput genomic DNA isolation.High Pure PCR Template Preparation Kit; Instructions For UseNucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.Note: A special Inhibitor Removal Buffer is included which allows the use of heparinized sample material (100 U/mL of heparin). This buffer increases the sensitivity and reproducibility of assays performed with the isolated nucleic acid, even when the sample contains heparin.Capacity: The High Pure Spin Filter Tubes hold up to 700 muL sample volume.
Application: The High Pure PCR Template Preparation Kit purifies nucleic acids from a wide variety of sample materials including whole blood, cultured cells, and tissue. The isolated nucleic acids can be used for:
•Long-template PCR
•Real-time, quantitative PCR
•SNP detection
•Southern blotting
•Cloning
Legal Information: LightCycler is a registered trademark of Roche
Other Notes: High Pure PCR Template Preparation KitRapid and Reliable Methylation Detection in Archival Tissue Samples using High-Resolution Melting Analysis