Synonyms: HET 0016; HET-0016; N'-(4-Butyl-2-methylphenyl)-N-hydroxymethanimidamide; N-(4-Butyl-2-methylphenyl)-N'-hydroxyformamidine; N-(4-Butyl-2-methylphenyl)-N'-hydroxymethanimidamide; N-Hydroxy-N'-(4-n-butyl-2-methylphenyl)formamidine
Molecular Formula: C12H18N2O
Molecular Weight: 206.28
Linear Structural Formula: C12H18N2O
Purity: >=95% (HPLC)
Storage: -20C
Biochem Physiol Actions: HET0016 is a potent and selective inhibitor against cytochrome P450 (CYP450) ω-hydroxylases (human CYP4A11/4A22/4F2/4F3, rat CYP4A1/4A2/4A3/4A8)-mediated 20-hydroxyeicosatetraenoic acid (20-HETE) biosynthesis (IC50 = 8.9/35 nM using human/rat renal microsomes), exhibiting much reduced potency against epoxyeicosatrienoic acids (EETs) biosynthesis (IC50 = 2.8 μM/rat microsome) or the enzyme activities of CYP2C9/2D6/3A4 and COX (IC50 = 3.3/83.9/71 μM and 2.3 μM). HET0016 is widely employed both in cultures (1-10 μM) and in aminals in vivo (1-10 mg/kg via i.v., i.m., i.p. or s.c.) for probing 20-HETE-dependent physiological and pathological processes.