Guanosine-5'-triphosphate, a purine nucleotide, is required as a coenzyme for protein biosynthesis in a cell-free system.
Guanosine 5′-triphosphate (GTP), a purine trinucleotide, is a substrate for RNA polymerases and a wide range of GTPase including G-protein coupled receptor (GPCR) GTPases and cell signaling and cycling associated guanine nucleotide exhange factors (GEF).
GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. GTP activates the signal transducing G proteins which are involved in various cellular processes including proliferation, differentiation, and activation of several intracellular kinase cascades. Proliferation and apoptosis are regulated in part by the hydrolysis of GTP by small GTPases Ras and Rho. Another type of small GTPase, Rab, plays a role in the docking and fusion of vesicles and may also be involved in vesicle formation. In addition to its role in signal transduction, GTP also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA.
Purity: ≥95%
Key Applications: Substrate for RNA polymerases
Application Areas: Molecular Biology
Product Type: Biochemicals
Biochemical Category: Nucleotides & Nucleic Acids
Chemical Class: Nucleotides & Nucleic Acids
Melting Point: 206 -207°C (Lit.)
Optical Rotation: [α]20/D −24±2°, c = 1% in 0.5 M Na2HPO4
UV/Visible Absorbance: λ max (water): 252 ± 3 nm
Presentation: White Powder
Format: Powder
Detection Method: FTIR
Solubility: Soluble in water (50 mg/mL - clear and colorless).
Storage & Handling: -20°C, desiccate