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MP Biomedicals

Goat Affinity Purified Antibody To Hamster IgG (whole Molecule)

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Goat Affinity Purified Antibody To Hamster IgG (whole Molecule)

Analysis Note
The total protein is measured by absorbance (A280nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat IgG; no trace of albumin is detected.

Preparation Method
The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No preservative has been added.

Applications
Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

Product Description
Product is goat affinity purified antibody to hamster IgG (whole molecule) and buffer salts.

Typical Working Concentration
Suggested working dilutions: 1:50-1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 2,000 for Elisa

Concentration: Total Protein: 1.0 mg/mL; Active Antibody: 1.0 mg/mL
Suitability:Goat affinity purified antibody to hamster IgG (whole molecule) is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.
Key Applications:ELISA, Immunohistochemical, Western Blotting, Immunoassays
Product Type:Antibodies
Antibody Type:Secondary Antibodies
Antibody Category:Polyclonal
Conjugate:Unconjugated
Host:Goat
Immunogen:Mouse IgG
Species Reactivity:It shows reactivity to hamster IgG; cross-reactivity to other species may exist. Because the antibody is directed against whole IgG, some antibodies are expected to bind light chain sites common to all immunoglobulins.
Format: affinity purified liquid
Storage & Handling:Store unopened product at 2-8 °C. Store opened material no longer than 1-2 days at 2-8 °C unless preservative is added. For long-term storage, aliquot and store with 40% glycerol (v/v) at -20 °C.

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Product Detail
Thomas No.
C837V20
Mfr. No.
0856984
Description
Goat Affinity Purified Antibody To Hamster IgG (whole Molecule)
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