This purified antibody has been tested for use in neutralizations, ELISA and western blotting. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be<10 pg micro;l by the lal method. by western blot a band approximately 14.5 kda in size corresponding to human gm-csf protein is expected in the appropriate cell lysate or extract. specific conditions for reactivity should be optimized by the end user.<>