Glial Cell Line-derived Neurotrophic Factor from rat recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, =97% (SDS-PAGE)

Synonym: ATF, Astrocyte-derived trophic factor, GDNF

MDL Number: MFCD01093875

Analysis Note
The biological activity of GDNF is measured by its ability to bind to immobilized rrGFRa1/Fc in a functional ELISA.

Physical Form
Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid (TFA) containing 50 μg bovine serum albumin (BSA) per 1 μg of cytokine.

Biochem/physiol Actions
Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that is a member of the TGF-β superfamily. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share 93% sequence homology with strong species cross-reactivity. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development including motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.