Gentamicin sulpahte is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1, C1a, and C2. Gentamicin is used as the sulfate salt. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base.
Gentamicin sulpahte is used as a selection agent (gentamicin-resistance gene) in molecular biology applications. It is used to treat many types of bacterial infections, particularly those caused by Gram-negative organisms.
Gentamicin causes codon misreading by binding to the 30S ribosomal subunit, blocking the translocation of peptidyl-tRNA from the acceptor site to the donor site. The bactericidal effect of gentamicin on Pseudomonas aeruginosa is exerted by the binding of gentamicin to the outer membrane, where it displaces natural cations, destabilizes the membrane, and forms holes in the cell surface. Antimicrobial spectrum: Gram-negative bacteria, Staphylococcus aureus and other Gram-positive bacteria.
Grade: USP Grade
Potency: ≥590 µg/mg
Key Applications: Aminoglycosides, Antibacterial
Product Type: Biochemicals
Biochemical Category: Antibiotics
Melting Point: 218-237°C (as powder) (Lit.)
Optical Rotation: +107° to +121°(10 mg/mL, water)
Presentation: White Powder
pH: 3.5 - 5.5 (1 in 25, in water)
Loss on Drying: ≤18.0 %
Residue on ignition: ≤1.0%
NOTES:
Limit of Methanol ≤1.0%
Solubility: H2O: soluble 50 mg/mL
Storage & Handling: Store at +4°C.