We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.
Application: Traditional methods for purifying genomic DNA from liquid blood samples >= 200 mul rely on medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low DNA concentration, and low purity. The GenElute(TM)-E Single Spin Blood DNA High Yield Kit, extracts up to 20 mug highly concentrated DNA (200 ng/mul) while offering the flexibility of sample inputs ranging from 200 mul to 1 ml liquid blood. EDTA-, citrate- as well as heparin-stabilized human and animal blood can be used. Enriched DNA yield and concentration make this kit well suited for researchers with limited or low-titer samples who require high sensitivity for NGS or other detection methods. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.
Legal Information: SmartLyse is a trademark of Merck KGaA, Darmstadt, Germany
Other Notes: We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. GenElute(TM)-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute(TM)-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute(TM)-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.