Cas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The design of single guide RNA (sgRNA) is dependent on the target region close to the PAM site. Even if you pick a target sequence that fulfills all of the described requirements, sgRNA specificity and activity is unpredictable. Therefore, it is often recommended that multiple, different sgRNAs be designed to target a gene of interest. The GenCrispr sgRNA Screening Kit provides a simple, reliable, and rapid method for assessing sgRNA efficiency before cell transduction, allowing you to identify the highly effective CRISPR sgRNA.
| Components | Amount |
| 30-reaction kit | 100-reaction kit |
| GenCrispr Cas9 Nuclease | 15 μL | 50 μL |
| 10X Reaction Buffer | 100 μL | 300 μL |
| Positive Control sgRNA | 3 μg | 10 μg |
| Positive Control Substrate | 60 μL | 200 μL |
| RNase-free water | 1 mL | 1 mL |