The GenCrispr Mutation Detection Kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic DNA that is extracted from cells transfected with constructs expressing engineered nucleases such as Transcription activator-like effector nucleases (TALEN), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, or Zinc-finger nucleases (ZFN). The GenCrispr Mutation Detection Kit includes High-Fidelity DNA polymerase for amplifying the target regions from cells, and GenCrispr T7 Endonuclease I for recognizing and detecting the mismatches caused by gene editing tools. It provides an easy and reliable approach for estimating the efficiency of genome editing.
| Components | Amount provided |
| 25-reaction kit | 100-reaction kit |
| High-Fidelity DNA polymerase | 10 μL | 40 μL |
| 5X PCR Reaction Buffer | 150 μL | 600 μL |
| GenCrispr T7 Endonuclease I (Cat.No.Z03396) | 25 μL | 100 μL |
| 10X GenCrispr T7 Endonuclease I Reaction buffer | 50 μL | 500 μL |
| Control Template DNA | 30 μL | 120 μL |
| Control Primer Mix | 30 μL | 120 μL |
| Protease K | 25 μL | 100 μL |
| dNTP | 15 μL | 60 μL |