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GenScript

GenCRISPR™ Cas13a (C2c2) Nuclease

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GenCRISPR Cas13a (C2c2) Nuclease is an RNA-guided, RNA-targeting CRISPR enzyme, commonly referred to as C2c2. This enzyme is a member of Type VI CRISPR family containing a single protein effector with two HEPN domains.  The Cas13a exhibits two RNase activities. The first activity, a cis- sequence-specific RNA guided cleavage, activates the secondary RNase activity of the enzyme, a non-specific trans- ribonuclease activity. This Cas13a protein can be applied to detect single RNA molecules with proven high specificity.

Recently, Cas13a has been leveraged as a diagnostic nucleic acid detection tool. Using a combination of the Cas13a cis- and trans- RNase activity and a non-target RNA tagged with a cleavage reporter ((fluorescence), the Cas13 system provides a route for reporting of the presence of a specific single RNA sequence in a sample with high specificity. The GenScript Cas13a contains a His-tag at the C-terminal.

Expression SystemRecombinant Cas13a expressed by E.coli
Accession#U2PSH1
SpeciesLeptotrichia wadei
Apparent Molecular Weight~140 kDa, on SDS-PAGE under non-reducing conditions
FormulationSupplied as a solution of 10 mM Tris, pH 8.0, 300 mM NaCl, 1.0 mM DTT, and 50% glycerol. 
Quality Control Specifications
AssaySpecifications
AppearanceClear, colorless liquid
Purity by SDS-PAGE≥ 95%
Residual DNaseUndetectable
Residual RNaseUndetectable
Endotoxin Level< 0.2 EU/μg of the protein by gel clotting method
ConcentrationPlease refer to the COA for the specific lot.
StorageThis product remains stable up to 12 months at -20?. Avoid repeated freeze-thaw cycles.
NoteFor laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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Thomas No.
CHM02D070
Mfr. No.
Z03486-100
Description
GenCRISPR™ Cas13a (C2c2) Nuclease,100ug
list price/quantitytotal
$0.00
Thomas No.
CHM02D071
Mfr. No.
Z03486-500
Description
GenCRISPR™ Cas13a (C2c2) Nuclease,500ug
list price/quantitytotal
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Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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