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MilliporeSigma

Formamide, BioUltra, for molecular biology, =99.5% (T)

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Synonym: Amide C1, Formic amide

CAS Number: 75-12-7

Linear Formula: HCONH2

Molecular Weight: 45.04

Beilstein Registry Number: 505995

EC Number: 200-842-0

MDL number: MFCD00007941

PubChem Substance ID: 24871294

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other Notes
For the preparation of formamide denaturing polyacrylamide gels1; In situ hybridization on human chromosomes2

Grade: for molecular biology

Vapor Density: 1.55 (vs air)

Vapor Pressure: 0.08 mmHg ( 20 °C) ; 30 mmHg ( 129 °C)

Product Line: BioUltra

Assay: ≥99.5% (T)

Autoignition Temp.: 932 °F

Impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected

Ign. Residue: ≤0.1%

Refractive Index: n20/D 1.447(lit.); n20/D 1.447

pH: 7-9 (25 °C, 1 M in H2O)

BP: 210 °C(lit.)

MP: 2-3 °C(lit.)

Solubility: H2O: soluble10 M at 20 °C, clear, colorless

Density: 1.134 g/mL at 25 °C(lit.)

Anion Traces: chloride (Cl-): ≤1 mg/kg; sulfate (SO42-): ≤10 mg/kg

Cation Traces: Ag: ≤5 mg/kg; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; NH4+: ≤1000 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: ≤1 mg/kg

λ: 10 M in H2O

UV Absorption: λ: 260 nm Amax: ≤0.4; λ: 280 nm Amax: ≤0.1

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Product Detail
Thomas No.
C974L42
Mfr. No.
47671-250ML-F
Description
Formamide, BioUltra, 250 mL
list price/quantitytotal
$0.00
Thomas No.
C974L43
Mfr. No.
47671-1L-F
Description
Formamide, BioUltra, 1 L
list price/quantitytotal
$0.00
Thomas No.
C974L44
Mfr. No.
47671-2.5L-F
Description
Formamide, BioUltra, 2.5 L
list price/quantitytotal
$0.00
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