2X Forget-Me-Not™ EvaGreen® qPCR Master Mix is a ready-to-use high performance dye-based master mix for qPCR and DNA melt curve analysis. The 2X Master Mix contains EvaGreen® dye, Cheetah™ HotStart Taq DNA Polymerase, dNTPs, and a low concentration of an inert blue dye, which allows the user to easily distinguish wells containing reaction mix from empty wells. For 2-color tracking, mix your template with Forget-Me-Not™ Template Buffer to see at a glance which wells have master mix or template. • Ready-to-use, just add primers and DNA template • Superior EvaGreen® Dye is more sensitive than SYBR® Green I and is compatible with HRM® • Prevent wasted samples and costly mistakes with Forget-Me-Not™ 2-color tracking • Formulated with Cheetah™ HotStart Taq for fast cycling protocols • Directly visualize amplification product fluorescence in a gel • No ROX or Separate ROX for use with any PCR instrument