This fluorescent labeled catecholamine neurotransmitter analog of dopamine (Product Name: DnsylD-1), with dansyl attached by a 5-carbon spacer arm to dopamine, provides a unique pharmacological tool. DnsylD-1 is a stable, receptor selective and high affinity pharmacological ligand that can be detected by fluorescent microscopy and FLIPR devices. Unlike recently reported synthesized fluorescent neurotransmitter analogs, DnsylD-1 contains the entire native dopamine structural moiety attached with the 5-carbon polylinker to the small fluorescence label dansyl. This structural modification minimizes steric hindrance, making DnsylD-1 an effective reagent for dopamine ligand binding and transporter uptake assays at namomolar concentrations. A large stokes shift between excitation and emission maxima (Ex:Em, 313:515 nm) of DnsylD-1 provides high signal to noise detection with minimal autofluorescence. The dansyl moiety also changes spectral characteristics on intermolecular contact, facilitating biophysical characterization of ligand-receptor interaction. DnsylD-1 is a suitable substitute for radioactive ligand.
Synonyms: Dansyl-C5-Dopamine
Purity: >=98% (HPLC)
Storage: 2-8C
Application: Applications for Fluorescent Dopamine Ligand-Neurotransmitter (DnsylD-1)DnsylD-1 has the following applicability due to the small molecular size of the dansyl fluorescent label, a long spacer arm between the dopamine structure and the fluorescent tag, stability in aqueous solution and low autofluorescence of the surrounding cellular or tissue medium.
•Histochemical imaging and quantitation of dopamine receptor expression in vivo and vitro.
•Pharmacological applications as a substitute for radioactive ligand.
•Dopamine metabolism and turnover analysis.
•Dopamine uptake analysis.
•Dopaminergic neuron detection.
•Analysis of disease conditions associated with altered dopamine synthesis, dopamine receptor expression or dopamine uptake: human disease research applicability in the following: a) Parkinson's disease. b) Psychological disorder, including ADHD, schizophrenia and substance abuse. c) Physiological regulation of cardiovascular, renal and immune function.
Other Notes: Proprietary product of Fivephoton Biochemicals