Fluorescein methotrexate is useful for studying anti-metabolite resistance and spontaneous gene amplification. The dye is actively taken up by cells and binds to dihydrofolate reductase (DHFR) quantitatively.
- λEx/λ Em = 496/516nm
- Yellow solid soluble in pH>6.5 buffer, or DMF
- Store desiccated at 4°C and protect from light
- C46H54N14O9S
- MW:979.08