Synonyms: Azure eosin methylene blue; Giemsa solution
CAS Number: 51811-82-6
MDL No.: MFCD00081642
EC Number: 200-659-6
UNSPSC Code: 12352200
Application: Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.
Physical form: Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)
Principle: When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
Reconstitution: Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry
RIDADR: UN1230 - class 3 - PG 2 - Methanol, solution
WGK Germany: 2
Flash Point(F): 48.2 F
Flash Point(C): 9 C
R Codes: 11-23/24/25-39/23/24/25
S Codes: 7-16-36/37-45
IOD Codes: F,T
Symbol: GHS02, GHS06, GHS08
Signal Word: Danger
Hazard Statements: H225-H301 + H311 + H331-H370
Precautionary statements: P210-P260-P280-P310