MDL No.: MFCD00163600
Storage: 2-8C
UNSPSC Code: 12352203
General description: Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin(R) is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.
Application: ExtrAvidin(TM) -Alkaline Phosphatase is suitable for the following applications:. Dot blot at a dilution of 1:300,000. Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100 using human tissues sections. Indirect ELISA (at a dilution of 1:60,000). ELISA. Enzyme-linked immunosorbent spot (ELISPOT) analysis. Competitive ELISA
Physical form: Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide
Preparation Note: Affinity purified protein
RIDADR: NONH for all modes of transport
WGK Germany: 2