MDL Number: MFCD01634638
Storage: 2-8C
Application: EX-CELL(R) 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or the GS System(TM), because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic(TM) F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.
Legal Information: EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC