Synonym: Carboxyl esterase, Carboxylic-ester hydrolase, PLE
CAS Number: 9016-18-6
EC Number: 232-773-7
Enzyme Commission (EC) Number: 3.1.1.1
MDL number: MFCD00131030
Application
Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.2
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.3,4
Esterase from porcine liver has been used in a study to assess the effect of 5-aminolaevulinic acid peptide prodrugs on photosensitization for photodynamic therapy. Esterase from porcine liver has also been used in a study to investigate how site-specific atherogenic gene expression correlates with subsequent variable lesion development in coronary and peripheral vasculature.
Unit Definition
One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C
Physical Form
Crude powder containing ≤10% buffer salts
Biochem/physiol Actions
Esterase acts on water-soluble carboxyl esters containing short chain fatty acids.1 Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.