Storage: -196C
UNSPSC Code: 12352200
Application: For complete product information, please see ECACC
Application: Recommended culture medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Cell Line Origin: Distal oesophageal adenocarcinoma,
Culture Medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
DNA Profile: STR-PCR Data: Amelogenin: X, CSF1PO: 10 D13S317: 11 D16S539: 13 D5S818: 11 D7S820: 8.2 THO1: 9 TPOX: 8 vWA: 14,15
Subculture Routine: Maintain cultures between 2-9 x 105 cells/cm3 by addition of an equal volume of fresh medium every other day. The DMSO must be removed by centrifugation of the cells at resuscitation. 5% CO2; 37C.
Other Notes: Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
RIDADR: NONH for all modes of transport