The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.
Synonyms: Murine LIF, Mouse LIF, mLIF
Purity: >95% (active component mLIF, SDS-PAGE)
Application: Applications for ESGRO Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells. SUGGESTED PROTOCOLS: ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U ESGRO Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3). At the recommended concentration 107 units of ESGRO is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO Supplement is sufficient for 1.0 L of tissue culture media.
Legal Information: ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany