ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3beta inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency.
Application: Suitable for ES cell derivation, transfection and selection, in addition to general cell passaging and growth. Media can be used on feeder dependent/independent ES cell lines. Important passaging information Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells using ESGRO Complete products. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures. Recommended products include: ESGRO Complete(TM) Accutase (Cat # SF006) ESGRO Complete Trypsin Solution (proprietary) (Cat # SF007) ESGRO Complete Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)
Legal Information: cOmplete is a trademark of Roche