For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
- Linear detection range 1 - 50 μM L-lactate in 96-well plate assay.
- Can be readily automated to test thousands of samples per day.
- Method: FL530/585 nm.
- Samples: Serum, plasma, cell culture media etc.
LACTATE is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems EnzyFluo™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the lactate concentration in the sample.