For quantitative determination of glutathione reductase activity.
- Linear detection range 0.4 to 50 U/L for 20 min reaction.
- Homogeneous "mix-incubate-measure" type assay.
- Can be readily automated to process thousands of samples per day.
- Method: OD412nm.
- Samples: Plasma, serum, tissue and culture media etc.
GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. BioAssay Systems? non-radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman?s method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample.