For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.
- Linear detection range 0.05 - 2 mM D-lactate in 96-well plate assay.
- Convenient room temperature assay. No 37°C heater is needed.
- Can be readily automated to process thousands of samples per day.
- Method: OD565 nm.
- Samples: Serum, plasma, cell culture media etc.
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.