For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.
- Linear detection range 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
- Can be readily automated to process thousands of samples per day.
- Method: OD570 nm, or FL530/585 nm.
- Samples: Serum, plasma, agriculture etc.
CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. BioAssay Systems? Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample.